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小鼠感染PCV-2的病理学研究(2)

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目    录
摘要……………………………………………………………………………1
关键词…………………………………………………………………………1
1前言……………………………………………………………………………2
2材料与方法……………………………………………………………………3
2.1 实验材料…………………………………………………………………………………3
2.1.1 PCV-2…………………………………………………………………………………3
2.1.2实验动物………………………………………………………………………………3
2.2实验仪器和试剂…………………………………………………………………………3
2.2.1 实验仪器………………………………………………………………………………3
2.2.2 试剂……………………………………………………………………………………3
2.3实验方法…………………………………………………………………………………4
2.3.1 实验动物感染方法……………………………………………………………………4
2.3.2小鼠临床症状及剖检眼观病变的观察………………………………………………4
2.3.3实验动物组织脏器的病理切片制作和观察…………………………………………4
3结果与分析………………………………………………………………………5
3.1临床症状…………………………………………………………………………………5
3.2剖检变化…………………………………………………………………………………5
3.3病理组织学变化…………………………………………………………………………5
4讨论……………………………………………………………………………6
参考文献 ………………………………………………………………………7
致谢 ………………………………………………………………………9
附表1 ………………………………………………………………………10

参考文献
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[3]尹业师,伟坚,刘海鹏,等. 猪2型圆环病毒核酸疫苗的研制及其对小白鼠的免疫效果[J].中国兽医科学2007(2):13
[4]Darwich L, Segales J, Mateu E. Pathogenesis of postweaning multisystemic wasting syndrome caused by porcine circovirus 2[J]. An immune riddle 2004(5):35
[5]Plana Duran, Balash M Segales. Veterinary Record [J] 1999 (5):76-77
[6]Quintana J, Balasch M, Segales J. Experimental inoculation of porcine circoviruses type 1 (PCV1) and type 2 (PCV2) in rabbits and mice [J]. Veterinary Record 2002(3):85
[7]崔尚金,全滟平,李曦,等. 猪圆环病毒间接免疫荧光方法的建立[J]. 中国预防兽医学报2007(1) :13
[8]温永俊,吴国军,蔡雪辉,等. 猪圆环病毒和猪繁殖与呼吸综合征病毒混合感染对仔猪致病性的评估[J]. 中国预防兽医学报2007(5):56
[9]赵妍,王君伟,邢明伟,等. 二重PCR检测鸭瘟病毒和鹅细小病毒的初步研究 [J]. 中国预防兽医学报2007(9):44
[10]Nayar, Hamel, Linn. Evidence for circovirus in cattle with respiratory disease and from aborted bovine fetuses [J]. 1999(4):232-233
[11]陈义祥,刘翠权,何丹,等.广西猪圆环病毒2 型感染的流行病学调查[J].中国兽医科技,2005,35(10):827-831.
[12]Rovira, Balasch. SEGALES [J]. Experimental inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus 2 2002(7):32
[13]Fenaux, Opriessnig, Halbur P G, et al. Immunogenicity and pathogen city of chimeric infectious DNA clones of pathogenic porcine circovirus type 2 ( PCV 2 ) and nonpatho2genic PCV 1 in weanling pigs[J ]. Journal of Viro logy, 2003, 77(20): 11232211243.
[14]Fenaux M , Halbur P G, Haqshenas G, et al. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathos logic lesions[J ]. Journal of Viro logy, 2002, 76 (2): 5412551.
[15]Allan GM , Mcneilly F, Meehan B M , et al. A sequential study of experimental infection of pigs with porcine circovirus and porcine parvovirus: immune staining of cry stat sections and virus isolation [J ]. J Vet Med, 2000, 47 (2): 81294.
[16] 陈义祥,刘翠权,何丹,等.广西猪圆环病毒2 型感染的流行病学调查[J].中国兽医科技,2005,35(10):827-831.
[17] Liu J, Chen I, Kwang J. Characterization of a previously unidentified viral protein in porcine circovirus type 2 -infected cells and its role in virus- induced apoptosis[J].J Virol, 2005,79(13):8262-8274.
[18] Kim J, Chung H K, Chae C. Association of porcine circovirus with porcine respiratory disease complex[J].Vet J,2003,166:251

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