福寿螺消化酶活性对其在浏阳河分布影响的初步研究

福寿螺消化酶活性对其在浏阳河分布影响的初步研究(毕业论文14000字)
摘  要：浏阳河有福寿螺河段全长160公里，并且逐年持续向下游扩散，通过
对福寿螺和中国圆田螺的消化酶活性进行比较分析，结果表明福寿螺的四种主要消化酶：纤维素酶、蛋白酶、淀粉酶、脂肪酶的活性都要高于同一生态位的中国圆田螺，在种间竞争中占优势，这些优势为福寿螺在浏阳河的繁殖扩散提供了条件，威胁本地生物多样性,应立即采取有效防治措施，阻止这一外来物种向湘江的扩散。
关键词：福寿螺；中国圆田螺；浏阳河；扩散；消化酶；预测

The Preliminary Study of the Activation of Pomacea canaliculata’s digestive enzyme activities that Distributed in Liu Yang River
Abstract：Having occupied the length of Liu Yang River for 160 miles, Pomacea canaliculata  continue to spread downriver year by year. The four major are cellulose, protease, protease and lipase. Through the comparative analysis of  Pomacea canaliculata  and Cipangopaludina chinensis,, it comes the result that all of the activity in these digestive enzymes are higher than Cipangopaludina chinensis,in the same ecological niche, which made them superior over the last one. And this superiority provide Pomacea canaliculata  the condition of breeding and spreading in Liu Yang River and threat the diversity of the native creatures. Therefore it is necessary to take some effective measures in order to prohibit this exotic species spreading into the Xiang Jiang River
Key words: Pomacea canaliculata; Cipangopaludina chinensis；Liu yang River; proliferation; digestive enzymes；forecast;

目   录  
摘要……………………………………………………………………………1
关键词…………………………………………………………………………1
1前言……………………………………………………………………………2
2 材料与方法……………………………………………………………………4
     2.1材料…………………………………………………………………4
          2.1.1福寿螺、田螺……………………………………………………4
          2.1.2试验地点、试验用土………………………………………4
          2.1.3福寿螺与田螺内脏粗酶液的制备……………………………4
          2.1.4福寿螺与田螺纤维素酶活力测定试验试剂……………………4
          2.1.5福寿螺与田螺蛋白酶活力测定试验试剂………………………5
          2.1.6福寿螺与田螺淀粉酶活力测定试验试剂………………………5
          2.1.7福寿螺与田螺脂肪酶活力测定试验试剂………………………6
          2.1.8 实验设备…………………………………………………………6
     2.2方法………………………………………………………………6
          2.2.1浏阳河福寿螺的分布调查研究方法……………………………7
          2.2.2福寿螺与田螺胃肠组织中纤维素酶活力的测定………………7
          2.2.3福寿螺与田螺胃肠组织中蛋白酶活力的测定…………………8
          2.2.4福寿螺与田螺胃肠组织中淀粉酶活力的测定…………………8
          2.2.5福寿螺与田螺胃肠组织中脂肪酶活力的测定…………………9
3结果与分析…………………………………………………………………………9
     3. 1 福寿螺在浏阳河的分布调查………………………………………10
     3. 2 福寿螺和田螺胃肠组织中纤维素酶活力的测定结果……………………11
     3. 3 福寿螺和田螺胃肠组织中蛋白酶活力的测定结果…………………11
     3. 4 福寿螺和田螺胃肠组织中淀粉酶活力的测定结果…………………12
     3. 5 福寿螺和田螺胃肠组织中脂肪酶活力的测定结果…………………13
4讨论………………………………………………………………………14
5 结论………………………………………………………………………16
参考文献………………………………………………………………………16
致谢……………………………………………………………………………18
附录…………………………………………………………………………18